D constructs with in vivo tissues. In the similar time, the

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D constructs with in vivo tissues. At the identical time, the limited and non-functional vascularization of thick cell sheet-based tissue engineering constructs after implantation has been tackled by co-culturing endothelial cells with other progenitor or committed cells. In reality, like for classic TE methods, the pre-vascularization of cell sheets-based constructs has been proposed as a method to circumvent this problem. In line with Rouwkema and colleagues, the pre-vascularization technique can d.D constructs with in vivo tissues. At the similar time, the limited and non-functional vascularization of thick cell sheet-based tissue engineering constructs just after implantation has been tackled by co-culturing endothelial cells with other progenitor or committed cells. In fact, like for standard TE methods, the pre-vascularization of cell sheets-based constructs has been proposed as a method to circumvent this problem. In accordance with Rouwkema and colleagues, the pre-vascularization method can d.